Polyethylene Glycol (PEG) is a polymer of ethylene oxide available in size variants from 400 Da to 40 kDa, PEG has a number of industrial and biomedical applications. In particular, therapeutic proteins are conjugated with PEG to slow down their clearance from circulation and improve bioavailability.

This antibody recognizes the repeating subunit (backbone) of Polyethylene Glycol (PEG). It reacts to various PEGylated proteins such as PEG-GCSF, PEG-Erythropoietin and PEG-Interferon, but does not react to unmodified form of the same proteins.

Western blot of PEGylated and unmodified versions of recombinant therapeutic proteins such as G-CSF, Interferon and Erythropoietin probed with Anti-PEG.

Product Highlights

1. Mouse monoclonal antibody (purified on Protein-G column)
2. Reacts to PEGylated recombinant proteins
3. Shipped in Lyophilized form (1 mg/ml concentration after reconstitution)
   

   Tested Applications

   Tested in Western Blot

   Amount of protein loaded : 200- 250 ng

   Antibody used: 1 μg/ml

   Tested in Indirect ELISA

   Antibody at 5 μg/ml concentration detects 100 ng/ml of PEGylated protein with OD > 1.

   Tested in Sandwich ELISA

   Detects a PEGylated protein in human serum (MRD 1:100) and plasma (MRD 1:10).

Ordering Information